Description

Cutaneous lymphocyte antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T cells in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells as well as on monocytes, granulocytes, and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on the majority of peripheral blood leukocytes. CLA is a ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
2. Picker LJ, et al. 1991. Nature 349:796. (IHC)
3. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
4. Borges E, et al. 1997. J. Biol. Chem. 272:28786.
5. Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed