Purified anti-Cytochrome c
Description
Cytochrome c is a 15 kD protein found in the mitochondrial intermembrane space with a heme-binding domain. Cytochrome c is a component of the electron transport chain; the heme group transfers electrons from cytochrome b-c1 complex to cytochrome oxidase complex. Cytochrome c initiates apoptosis by release to cytoplasm and binding Apaf-1 which activates procaspase 9. Cytochrome c interacts with the cytochrome b-c1 complex, cytochrome oxidase complex, heme, Apaf-1, and Caspase 9 proteins. The 6H2.B4 monoclonal antibody recognizes human, mouse, and rat cytochrome-c and has been shown to be useful for intracellular flow cytometric staining, Western blotting, immunoprecipitation, and immunofluorescence staining.
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.Recommended Usage
Each lot of this antibody is quality control tested . This antibody can be used at 2-4 microg /1 x107 cell equivalents for immunoprecipitation. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per 106 cells in 100 microL volume.
It is recommended that the reagent be titrated for optimal performance for each application.
References
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