Description

Streptavidin binds to biotin with high affinity. Streptavidin-PerCP is useful for detecting biotinylated antibodies. The excitation of PerCP by 488 nm laser light induces a light emission maximum of 675 nm.

Formulation

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

References

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7. Tsiantoulas D, et al. 2015. J Lipid Res. 56:440. PubMed