PerCP/Cy5.5 anti-BrdU

Antibodies Single
Sony
3D4
Flow Cytometry
Mouse IgG1, κ
Iodouridine-conjugated ovalbumin
2420550
$341.00

Description

BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

References

1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
3. Godebu E, et al. 2008. J. Immunol. 181:1798.
4. Waskow C, et al. 2008. Nat. Immunol. 9:676.