Description

CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 10⁶ cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Maeda, K., et al. 1997. Int. Immunol. 9:993. (IP, Block)
2. Bluestone, J.A. 1995. Immunity 2:555. (IP, Block)
3. Damoiseaux, J.G., et al. 1998. J. Leuk. Biol. 64:803. (IP, Block, IHC)
4. Zonneveld-Huijssoon E, et al. 2012. Ann Rheum Dis. PubMed.
5. Treacy O, et al. 2012. PLoS One. 7:e42662. PubMed.
6. Zonneveld-Huijssoon E, et al. 2012. Ann Rheum Dis. 71:1706. PubMed.