Pacific Blue™ anti-mouse GM-CSF

Antibodies Single
Sony
MP1-22E9
Intracellular Flow Cytometry
Rat IgG2a, κ
Mouse
Yeast-expressed, recombinant mouse GM-CSF
3127125
$128.00

Description

GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils, and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

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  2. Suda T, et al. 1990. Cell. Immunol. 129:228.
  3. Nozaki S, et al. 1991. J. Invest. Dermatol. 97:10.
  4. Abrams JS, et al. 1992. Immunol. Rev. 127:5.
  5. Abrams JS. 2001. Curr. Protoc. Immunol. Unit 6.20.
  6. Sander B, et al. 1991. Immunol. Rev. 119:65.
  7. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  8. Larkin J, et al. 2006. J. Immunol. 177:268.