Description

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

This reagent is developed for immunofluorescent staining for flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Cervantes-Barragan L, et al. 2007. Blood 109:1131.
2. Zeiser R, et al. 2007. Blood 109:2225.
3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed
4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed
5. Yi H, et al. 2009. Blood 113:5819. PubMed
6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed