Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca²⁺ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 microg per 10⁶ cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
6. Hardy B, et al. 2005. Int. Immunol. 17:615.
7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
9. Dow LE, et al. 2014. PLoS One. 9:95236. PubMed