Mouse Regulatory B Cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647)

Cocktail
Sony
6D5, 53-7.3, 1B1
Flow Cytometry
Rat IgG2a,Rat IgG2b
Mouse
25 tests
1263005
$358.00

Description

Splenic IL-10-producing CD1dhighCD5+ B cells have been designated as regulatory B cells (Breg or B10). These rare IL-10- producing B cells represent only 1%-2% of spleen B cells and 7%-8% of peritoneal B cells in mice but are not normally detectable in blood or lymph nodes. B10 cells are a unique subset of potent regulatory B cells different from B-1a, B-1b, or CD21+CD23- mantle zone B cells. B10 cells produce IL-10 and negatively regulate T cell function. Mouse Regulatory B Cell Flow Kit is composed of fluorochrome conjugated anti-mouse CD19, CD5, and CD1d. It is optimized for detection or isolation of B10 cells by flow cytometry.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 microL per million cells.

Materials provided:
FITC anti-mouse CD19/PE anti-mouse CD5/Alexa Fluor® 647 anti-mouse CD1d, 25 tests
FITC Rat IgG2a/PE Rat IgG2a/Alexa Fluor® 647 Rat IgG2b isotype controls, 25 tests

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)
TruStain FcX (Cat. No. 101319)
Single color compensation controls