SP6800 Spectral Analyzer

instrument specifications

SP6800 instrument specification
Instrument
Configuration 2 laser model, 488/638 2 laser model, 405/488 3 laser model, 405/488/638
Model Number LE-SP6800ZB LE-SP6800ZC LE-SP6800ZE
Optics
Laser Specification
Lasers Semiconductor 2 laser model
488nm, 638nm
Semiconductor 2 laser model
405/488
Semiconductor 3 laser model
405/488/638
Maximum power (at flow cell) 488nm 40mW
638nm 60mW
405nm 60mW
488nm 40mW
405nm 60mW, 488nm 40mW
638nm 60mW
Detection Optics
Scatter signals Forward scatter (FSC), Side scatter (SSC)
Spectroscopic method Prism array spectroscopy
Fluorescent channels 32 channel PMT (wavelength: 500-800nm) 32 channel PMT (wavelength: 500-800nm) PMT x 2(420-440nm, 450-470nm)
System
Signal Resolution Height 20 bit, Area 32 bit Sampling frequency: 50MHz
Measurement parameters 64 channels, FSC, SSC,
cell position XY
66 channels, FSC, SSC, cell position XY
Pulse shape parameters Height, Area, Width
Optical alignment Automated alignment with Corefinder™ technology
Event Rate 10,000eps (standard)/20,000eps (maximum)
Fluidics
Sample loader type Single auto-loader
Supporting sample tube 5ml polystyrene round tubes
Sheath Flow Speed Low (approx. 3m/s), Mid (approx. 5m/s), High (approx. 10m/s)
Detection channel dimensions 200μm x 200μm
Performance
Fluorescence sensitivity FITC: 120 MESF / PE: 70 MESF
Linearity FITC R2 ≧0.995 / PE R2 ≧0.995
Detection size range 0.5~40μm (beads)
Fluorescence detection resolution 488nm Laser/CH16, 638nm Laser/CH27* : ≧2.5% (HPCV)
Software
Unmixing algorithms Spectrum method (LSM, WLSM, PSA and Constraint option), Reverse matrix method (Conventional)
Autofluorescence Autofluorescence spectral detection
Virtual Filter Possible to change wavelength region for each fluorochromes in analysis
Export Data Format Flow Cytometry Standard (FCS) 3.0, 3.1
Main Unit
Dimensions Main unit: Width 60.0cm x Depth 63.5cm x Height 71.3cm
Fluidics cart: Width 78.6cm x Depth 52.1cm x Height 58.0cm

Weight Main unit: approx. 99kg (dried weight)/Fluidics cart: approx. 32kg (dried weight)
Air pressure supply 350kPa~450kPa (51psi~65psi)
AC Power supply AC100V 50/60Hz, AC120V 60Hz
Power consumption 220W (max)
Operating temperature 16-29 degrees Celsius, Room temperature variation: within 5 degrees Celsius
Operating humidity 20% to 80% (condensation free)
Recommended PC SP6800 workstation

instrument specifications

*Except for LE-SP6800ZC (This HPCV value is calculated for red laser)