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SP6800 Spectral Analyzer
instrument specifications
SP6800 instrument specification
| Instrument |
| Configuration |
2 laser model, 488/638 |
2 laser model, 405/488 |
3 laser model, 405/488/638 |
| Model Number |
LE-SP6800ZB |
LE-SP6800ZC |
LE-SP6800ZE |
|
| Optics |
| Laser Specification |
| Lasers |
Semiconductor 2 laser model
488nm, 638nm |
Semiconductor 2 laser model
405/488 |
Semiconductor 3 laser model
405/488/638 |
| Maximum power (at flow cell) |
488nm 40mW
638nm 60mW |
405nm 60mW
488nm 40mW |
405nm 60mW, 488nm 40mW
638nm 60mW |
| Detection Optics |
| Scatter signals |
Forward scatter (FSC), Side scatter (SSC) |
| Spectroscopic method |
Prism array spectroscopy |
| Fluorescent channels |
32 channel PMT (wavelength: 500-800nm) |
32 channel PMT (wavelength: 500-800nm) PMT x 2(420-440nm, 450-470nm) |
|
| System |
| Signal Resolution |
Height 20 bit, Area 32 bit Sampling frequency: 50MHz |
| Measurement parameters |
64 channels, FSC, SSC,
cell position XY |
66 channels, FSC, SSC, cell position XY |
| Pulse shape parameters |
Height, Area, Width |
| Optical alignment |
Automated alignment with Corefinder™ technology |
| Event Rate |
10,000eps (standard)/20,000eps (maximum) |
|
| Fluidics |
| Sample loader type |
Single auto-loader |
| Supporting sample tube |
5ml polystyrene round tubes |
| Sheath Flow Speed |
Low (approx. 3m/s), Mid (approx. 5m/s), High (approx. 10m/s) |
| Detection channel dimensions |
200μm x 200μm |
|
| Performance |
| Fluorescence sensitivity |
FITC: 120 MESF / PE: 70 MESF |
| Linearity |
FITC R2 ≧0.995 / PE R2 ≧0.995 |
| Detection size range |
0.5~40μm (beads) |
| Fluorescence detection resolution |
488nm Laser/CH16, 638nm Laser/CH27* : ≧2.5% (HPCV) |
|
| Software |
| Unmixing algorithms |
Spectrum method (LSM, WLSM, PSA and Constraint option), Reverse matrix method (Conventional) |
| Autofluorescence |
Autofluorescence spectral detection |
| Virtual Filter |
Possible to change wavelength region for each fluorochromes in analysis |
| Export Data Format |
Flow Cytometry Standard (FCS) 3.0, 3.1 |
|
| Main Unit |
| Dimensions |
Main unit: Width 60.0cm x Depth 63.5cm x Height 71.3cm
Fluidics cart: Width 78.6cm x Depth 52.1cm x Height 58.0cm
|
| Weight |
Main unit: approx. 99kg (dried weight)/Fluidics cart: approx. 32kg (dried weight) |
| Air pressure supply |
350kPa~450kPa (51psi~65psi) |
| AC Power supply |
AC100V 50/60Hz, AC120V 60Hz |
| Power consumption |
220W (max) |
| Operating temperature |
16-29 degrees Celsius, Room temperature variation: within 5 degrees Celsius |
| Operating humidity |
20% to 80% (condensation free) |
| Recommended PC |
SP6800 workstation |
|
instrument specifications
*Except for LE-SP6800ZC (This HPCV value is calculated for red laser)
