SP6800 Spectral Analyzer
16 color panel on Human Peripheral Blood
Application data using 2 lasers
Fluorochromes: 488nm Laser
CD3 | Alexa Fluor 488 |
CD8 | PE |
CD4 | PE Dazzle 594 |
CD24 | PE Alexa Fluor 610 |
CD19 | PE Cy5 |
CD20 | PE Cy5.5 |
CD13 | PerCP efluor® 710 |
CD45 | PE Cy7 |
Fluorochromes: 405nm Laser
HLA-DR | Pacific Blue |
CD38 | BV421 |
CD45RA | BV510 |
CD33 | BV570 |
CD16 | BV605 |
CD11b | BV650 |
CD11c | BV711 |
CD14 | BV785 |
Examples of the resolution of several important cell populations using only two lasers with the SP6800. Clear population resolution is obtained with highly overlapping fluorochomes such as PE-Cy5/PE-Cy5.5 (G) and Pacific Blue/BV421 (H).
16 color stained sample of Human Peripheral Blood
Application data using 3 lasers
Fluorochromes: 405nm Laser
HLA-DR | Pacific Blue |
CD5 | BV421 |
CD56 | BV510 |
CD33 | BV570 |
CD16 | BV605 |
Fluorochromes: 488nm Laser
CD3 | Alexa Fluor 488 |
CD8 | PE |
CD4 | PE Dazzle 594 |
CD24 | PE Alexa Fluor 610 |
CD19 | PE Cy5 |
CD20 | PE Cy5.5 |
CD13 | PerCP efluor® 710 |
CD45 | PE Cy7 |
Fluorochromes: 638nm Laser
CD38 | APC |
C11c | Alexa Fluor 700 |
CD45RA | APC-Cy7 |
Example of the detection of several important cell populations using sixteen fluorochromes excited by three lasers. Unlike conventional flow cytometers that can detect up to five off the blue, in this example we demonstrate that with the SP6800 eight fluorochromes can be excited off the blue with clear sample resolution. Even highly overlapping fluorchromes such as PE-Cy5 and PE-Cy5.5 can be resolved.
Application Data: Brilliant Violet Dyes
BV421 | BV605 |
BV510 | BV650 |
BV570 | BV711 |
BV785 |
Sample data from Human PBMCs stained with seven markers conjugated to Brilliant Violet dyes offered by Sony Biotechnology. D. All populations were clearly resolved including fluorochomes with significant spectral overlap such as BV605 and BV650 (D).
12-color Staining of Human Peripheral Blood Leukocytes
Example 1
Example 2
Example 3
Example 4
Example 5
Application Data: Dyes with issues
Example 1: Pacific Blue (457nm emission) and Brilliant Violet 421 (422nm emission)
Example 2: PE Cy5 (excited with 488nm and 638nm lasers) and APC (excited with 638nm laser)
Example 3: Fluorescent GFP and YFP need no special bandpass filter set with the SP6800
Example 4: PE Cy7 tandem is prone to falling apart causing issues with resolution when using conventional flow cytometers. Since Spectral Analysis unmixes the spectral fingerprint, the SP6800 produces excellent resolution.