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SH800S Cell Sorter

instrument specifications

SH800S cell sorter instrument specification
Optics
Excitation lasers 488 nm, 405 nm, 638 nm and 561 nm laser
Output power 30 mW (max.) optical fiber output
Beam alignment Collinear optical system
Detection parameters 6 fluorescence + 2 scatter
Analog-to-digital converters (ADC) 8-channel 20-bit, 110MHz
Pulse measurement Height, Area, Width
Fluidics
Sample tube Single, auto-loading tube
Tube types 0.5-mL, 1.5-mL, 5-mL, and 15-mL tubes
Sort devices 2-way tube, multiwell plates, PCR tubes, slides
Temperature control 5°C, 37°C (electric cooling method)
Agitation unit Eccentric rotation
Magnetic drive 300-rpm speed
Sorting chip size 70 μm, 100 μm and 130 μm
Sort Performance
Event rate 100,000 eps
Sorting speed Using the 70-μm sorting chip at 50 kHz, an average threshold of 12,000 events per second can be achieved with >98% purity and >80% yield. A threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.
Scatter resolution 0.5 μm
Fluorescence resolution < 2.5% half-peak coefficient of variation (HPCV)
Fluorescence sensitivity FITC 120 MESF, PE 110 MESF
Ancillary
Dimensions W: 21.7 in. (55 cm)x D: 21.7 in. (55 cm)x H: 28.4 in. (72 cm)
Fluidics cart W: 30 in. (78.6 cm) x 20.5 in. (52.1 cm) x 22.8 in. (58.0 cm)
Weight 216 lb (98 kg)
Fluidics cart 71 lb (32 kg) (dry weight)
LCD panel 7-inch, 800 x 480 pixels
Power supply 100-240 V, 50/60 Hz
Power consumption 500 W (max.)
Operating temperature 17.5°C to 27.5°C
Relative humidity 20% to 80%
Compliance:
Environmental
Rohs
Operating system Microsoft® Windows® 8 Professional, 64 bit
Data file structure Flow Cytometry Standard (FCS) 3.0 or 3.1
Safety standards compliance UL, CE, CSA

instrument specifications

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Fluorochrome Guide

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