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Overview
The benchtop SH800S cell sorter permits sorting of a wide range of cell sizes for many applications using the 70-μm, 100-μm, and 130-μm microfluidic sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to perform.
System software is intuitive and supports sorting into tubes and 96- and 384-well plates. The software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools.
Product Highlights
System Overview
The SH800S provides the highest level of automation and ease of use available in a cell sorter. Researchers can accurately set up, calibrate, and monitor sorting with a push of a button. This delivers true ease of operation to save time and improve consistency of results. A novel microfluidic sorting chip uses patented CoreFinder™ technology to automate key steps of instrument setup and operation, streamlining workflow. The system is versatile, allowing researchers to sort of a wide range of cell sizes to accommodate application requirements. Chip sizes include 70 μm, 100 μm, and 130 μm
Robust Fluidics
Central to the design of the SH800 Cell Sorter is a disposable microfluidic sorting chip that integrates the flow cell and nozzle into a single, user-friendly unit. Available in 70 μm, 100 μm, and 130 μm sizes, these chips support a wide range of cell types and sample conditions, enabling flexible sorting across diverse applications.
Consistent, Reliable Performance
The SH800’s microfluidic chip-based architecture is fundamental to delivering consistent and reproducible sorting results. By simplifying the fluidic path compared to conventional droplet sorters, the system reduces variability and operator-dependent setup.
This integrated design minimizes the risk of clogging and eliminates the need for complex alignment, enabling smooth, dependable operation with less downtime and greater day-to-day productivity in the lab.
Intuitive Operation
The SH800 is designed to make cell sorting straightforward from the moment you turn it on. Automated startup, alignment, and system checks reduce preparation time and remove the need for manual adjustments, allowing users to move quickly from sample to data with confidence.
Its intuitive software simplifies experiment creation through guided setup and preconfigured workflows, making it easy to define parameters, select sort conditions, and optimize for purity, yield, or single-cell applications. By minimizing complexity at every step, the SH800 enables users of all experience levels to set up experiments quickly and correctly.
During sorting, the system maintains this ease of use with streamlined controls and reliable performance, supporting everything from routine sorts to plate-based single-cell workflows. The result is a highly accessible sorting experience that helps labs stay productive while delivering consistent, high-quality results.
System Startup
Upon start up, the system initialization includes diagnostics that ensure all subsystems are properly working. Once verified, the system status and a green ready message are displayed on the LCD monitor on the front of the instrument.
Experiment Settings and Data Analysis
Users can create an experiment by selecting a new template, a recent experiment, or a shared template (public) from the Experiment window. If a new template is selected, dialogs guide the user to choose experiment settings such as sample groups, tubes, and pulse parameters for data acquisition. Once a template is selected, the user can start the acquisition or compensation wizard.
Data is displayed as dot plots and histograms on worksheets, and events can be marked using gates. The software has a number of tools to select, adjust, label, and measure statistics of target populations. Data can be easily exported as FCS formats (3.0 or 3.1 ) to use with third party analysis software.
Streamlined Workflows
Software, sensors, and CoreFinder patented technology provide automation across the workflow from set-up to shutdown to ensure consistency, save time and improve the accuracy of results. The interface is designed with ribbon tabs that logically organize features to make them rapidly accessible. An experiment-centric approach makes the software easy to teach, learn, and use.
Software wizards include step-wise workflows that guide users through start up, multicolor compensation, sorting, and system cleaning. Since the system simplifies even advanced sorting techniques, it can be used by researchers with little sorting experience.
Automated Chip Loading and Positioning
System set-up begins with a one-touch install to load the microfluidics flow cell chip. Actuators ensure precise positioning of the chip inside the chip loader. Sheath, sample, and vacuum lines connect and seal automatically to their respective ports once the chip is loaded. Made of durable plastic, the chip is easy to replace when needed.
Automated Side Stream Calibration
The angle and the position of the side streams are calculated and adjusted during setup for tube and plate sorting. This ensures that the sort stream is centered in the collection tube automatically without manual adjustment.
Automated Sort Monitoring and Droplet Calibration
The SH800S software monitors and actively makes adjustments to the drop drive to maintain a stable breakoff point. This feature ensures consistently good sort performance and allows detection of clogs and empty tubes, and facilitates walk-away operation.
The droplets are automatically calibrated by adjusting the frequency and the drop drive to achieve optimal break off point (BOP) for each type of sorting chip.