Overview
The ID7000™ Spectral Cell Analyzer delivers precision and flexibility for a wide range of experimental needs – from simple panels to complex, high-parameter studies. Its advanced optics feature a diffraction grating and 32-channel PMTs, enabling consistent, full-spectrum detection. Sony’s Weighted Least Squares Method (WLSM) ensures accurate unmixing and reduced spillover. With support for up to 8 autofluorescence parameters, the ID7000 provides enhanced clarity – even in autofluorescent samples. No matter the application, the ID7000 has the tools to adapt to your workflow and grow with your research.
The Spectral Flow Analysis (SFA) - Life Sciences Cloud Platform is a cloud-based solution that can efficiently perform a wide array of analysis protocols.
Product Highlights
ID7000 System Overview
The ID7000 is Sony’s most advanced spectral analyzer, capable of supporting everything from basic immunophenotyping to complex high-parameter panels. With up to 7 lasers and 186 detectors—the most of any commercially available system—the ID7000 offers unmatched detection capacity and a broad excitation range (320–808 nm), supporting 44+ color panels and compatibility with emerging fluorochromes. Intuitive software, automated setup and QC, and a powerful Spectral Reference Library streamline workflows, reducing the need for repeated single-color controls. The Autofluorescence Finder improves resolution in complex tissues, while the built-in AutoSampler enables walkaway operation across multiple plate formats. Purpose-built for precision, scalability, and ease of use, the ID7000 sets the standard for modern spectral flow cytometry.
High Capability Optics
The ID7000 features a diffraction grating–based optical system that evenly disperses emitted light across 32-channel PMT arrays, capturing continuous spectral data at ~10 nm resolution. This high resolution enables precise signal separation, minimizes spectral overlap, and improves unmixing performance. With a broad detection range, the system supports a wide variety of fluorochromes including newly developed dyes without requiring hardware changes, delivering cleaner data, greater flexibility, and confidence in every channel.
Key Features:
- Supports up to seven spatially separated lasers, with 320nm, 355nm, 405 nm, 488 nm, 561nm, 637 nm and 808 nm.
- Up to 186 detectors, including infrared-sensitive PMTs in a 7-laser configuration
- Advanced diffraction grating design maximizes photon capture from 494 to 844 nm, distributing light evenly across each 32-channel PMT array for consistent ~10 nm resolution
- Modular detection decks enable independent installation and straightforward field upgrades
- High-powered 405 nm laser paired with a sensitive detector enhances side scatter detection, enabling reliable identification of submicron particles down to ~80 nm
The ID7000 Spectral Cell Analyzer can detect wavelengths from 360 nm to 920 nm in the 7-laser configuration. The signal is captured using a combination of 32-channel PMT arrays and individual PMTs. The signal above 800 nm is detected with specialized InGaAs PMTs to ensure data quality.
High-Resolution Spectral Profiles
This plot shows spectral signatures collected across multiple lasers using the ID7000’s 32-channel PMT arrays. With ~10nm resolution and the WLSM unmixing algorithm, the system accurately separates closely spaced fluorochromes - enabling reliable detection of dim and overlapping markers across complex panels.
Efficient Operation
High-throughput AutoSampler
Data Quality
The ID7000 is engineered to distinguish even closely overlapping fluorochromes with confidence. Its powerful WLSM algorithm precisely separates fluorophores with similar emission profiles, minimizing spillover and improving resolution in densely populated panels. The system’s fine spectral resolution is further enhanced by the 320 nm deep UV laser, which adds 35 additional detection channels from 360 nm to 840 nm. Together, these innovations ensure accurate identification of dim, rare, and spectrally adjacent markers in any panel.
High Resolution Insights with the SFA Platform
This FIt-SNE visualization - generated using the SFA Life Sciences Cloud Platform - illustrates how the ID7000’s clean, well-separated data enables effective dimensionality reduction and clustering. With precise spectral unmixing and support for autofluorescence management, the ID7000 lays the foundation for high-confidence downstream analysis of complex cell populations.
Flexible Workflows
Acquisition to Analysis
The ID7000 supports a complete spectral workflow—from high-resolution acquisition to in-depth data analysis. Data collected on the system can be seamlessly imported to the Spectral Flow Analysis (SFA), Life Sciences Cloud Platform. SFA offers dimensionality reduction and clustering algorithms to uncover patterns in complex datasets, helping researchers visualize rare populations, explore heterogeneity, and draw deeper biological insights. Together, the ID7000 and SFA enable an end-to-end workflow that scales from routine assays to high-dimensional discovery. Learn more about SFA.
High Resolution Insights with the SFA Platform
Data visualization using UMAP and BL-FlowSOM.