FX500 Exchangeable Fluidics Cell Sorter

Instrument Specifications

Instrument Specifications
Excitation lasers 488-nm, 638-nm and 561-nm lasers
Output power 15 mW (max.) optical fiber output
Beam alignment Collinear optical system
Detection Parameters 8
Analog-to-digital converters (ADC) 8-channel 20-bit, 110 MHz
Pulse measurement Height, Area, Width
Sample tube Single, auto-loading tube
Tube types 0.5 mL, 1.5 mL, 5 mL and 15 mL tubes
Sort Devices 2-way tube, multiwell plates, PCR tubes, slides
Temperature control 5°C, 37°C (Electric Cooling Method)
Agitation unit Eccentric rotation
Magnetic drive 300 rpm speed
Sorting chip size 70 μm and 100 μm
Sort Performance
Sort Performance
Event rate 100,000 eps
Sorting Speed Using the 70-μm sorting chip at 50 kHz, an average threshold of 12,000 events per second can be achieved with >98% purity and >80% yield. The threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.
Scatter resolution 0.5 μm
Fluorescence resolution < 2.5% half-peak coefficient of variation (HPCV)
Fluorescence sensitivity FITC 120 MESF, PE 110 MESF
Sorting recovery >80% of Poisson's expected yield at 12,000 eps with 70µm sorting chip
Sorting purity >99% purity for using 70µm sorting chip
Dimensions W: 21.7 in. (55 cm)x D: 21.7 in. (55 cm)x H: 28.4 in. (72 cm)
Fluidics Cart W: 28.3 in. (71.5 cm) x 13.6 in. (34.5 cm) x 27.8 in. (70.5 cm)
Weight 220.5 lb (100 kg)
Fluidics Cart 81 lb (37 kg) (dry weight)
LCD panel 7-inch, 800 x 480 pixels
Power supply 100 V 50/60 Hz, 120 V 60 Hz
Power consumption 500 W (max.)
Operating temperature 17.5°C to 27.5°C
Relative humidity 20% to 80%
Operating System Microsoft® Windows® 10 Professional, 64 bit
Data File Structure Flow Cytometry Standard (FCS) 3.0 or 3.1
Safety Standards Compliance UL, CE, CSA

instrument specifications

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