FITC anti-mouse Lineage Cocktail with Isotype Ctrl

Cocktail
Sony
145-2C11, RB6-8C5, M1/70, RA3-6B2,Ter-119
Flow Cytometry
Hamster IgG,Rat IgG2a,Rat IgG2b
Mouse
1266505
$376.00

Description

The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The FITC Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3e, clone 145-2C11; anti-mouse Ly-6G/Ly-6C, clone RB6-8C5; anti-mouse CD11b, clone M1/70; anti-mouse CD45R/B220, clone RA3-6B2; anti-mouse TER-119/Erythroid cells, clone Ter-119. FITC Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin.

 

Materials provided:

  1. FITC anti-mCD3/ FITC anti-mGr-1/ FITC anti-mCD11b/ FITC anti-mCD45R(B220)/ FITC anti-mTer-119, 25 or 100 tests
  2. FITC Armenian hamster IgG/ FITC Rat IgG2b/ FITC Rat IgG2a isotype controls, 25 or 100 tests

Materials not provided:

Cell Staining Buffer (Cat. No. 420201)

Formulation

This two-reagent set contains FITC conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.

Recommended Usage

Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 microL per million cells or 20 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Morris SJ, et al. 1997. Development. 124(10):1929-1939
2. Okada S, et al. 1992. Blood180 (12):3044-3050
3. Spangrude GJ, et al. 1988. Science 241 (4861):58-62
4. Spangrude GJ, et al. 1990. Exp Hematol. 18 (8):920-926
5. Tan SL, et al. 2006. J. Immunol. 176:2872. PubMed
6. Chen G, et al. 2014. Cancer Immunol Res. 2:949. PubMed