DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity)
Description
This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (κ and λ) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may has minimal cross-reaction with other subclasses of mouse immunoglobulins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* DyLight™ 649 has a maximum absorption of 655nm and a maximum emission of 670nm (similar to Alexa Fluor® 647 and APC).
References
1. Scotta, C, et al. 2008. J. Immunol. 181:1025. PubMed