Description

IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The MP2-8F8 antibody can neutralize the bioactivity of natural or recombinant IL-3.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
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5. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20.
6. Finkelman, F., et al. 1993. J. Immunol. 151:1235.
7. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
8. Karulin, A., et al. 2000. J. Immunol. 164:1862.