Description

BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
3. Godebu E, et al. 2008. J. Immunol. 181:1798.
4. Waskow C, et al. 2008. Nat. Immunol. 9:676.