PE/Dazzle™ 594 anti-human CD163

Antibodies Single
Sony
GHI/61
Flow Cytometry
Mouse IgG1, κ
Human
2268120
$370.00

Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

References

1. Pulford K, et al. 1992. Immunology 75:588. (ICC, IP, WB)
2. Law SK, et al. 1993. Eur. J. Immunol. 23:2320.
3. Madsen M, et al. 2004. J. Biol. Chem. 279:51561.
4. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
5. Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed