Alexa Fluor® 594 anti-human Granzyme A
Description
Granzyme A is a 28 kD disulfide-linked homodimeric protein and the most abundant of the proteases occurring in CTL granules. It is homologous to other serine esterases, including other granyzmes, mast cell proteases, and neutrophil cathepsins. Granzyme B is thought to be a rapidly-acting apoptotic enzyme, while Granzyme A is slow acting.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescence staining. For immunofluorescence microscopy, a concentration range of 1-5 μg/ml is recommended. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
References
1. Trimble L, et al. 1998. Blood 91:585.
2. Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
3. Raqib R, et al. 2002. Infect. Immun. 70:3199.
4. Chen H, et al. 2005. J. Immunol. 175:591.