Brilliant Violet 570™ anti-human CD4

Antibodies Single
Sony
RPA-T4
Flow Cytometry
Mouse IgG1, κ
Human
2102670
$451.00

Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
6. Lan RY, et al. 2006. Hepatology 43:729.
7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)