PE/Cy7 anti-mouse CD49d

Antibodies Single
Sony
R1-2
Flow Cytometry
Rat IgG2b, κ
Mouse
AKR/Cum mouse spontaneous T lymphoma line TK1
1118085
$145.00

Description

CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
2. Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
3. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
4. Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
5. Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)