PE/Cy5 anti-human CD29

Antibodies Single
Sony
TS2/16
Flow Cytometry
Mouse IgG1, κ
Human
2115030
$290.00

Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)