Mouse Naïve/Memory T cell ID Panel
Description
In mice, CD4+ T cells can be divided into naïve and memory cells based on expression of adhesion molecules CD44 and CD62L. Naïve T cells exhibit high levels of CD62L and low expression of CD44, whereas memory T cells are identified by high CD44 and low CD62L expression. This panel includes antibodies against CD3, CD4, CD62L and CD44, which can be used together to identify naïve and memory T cell subsets in mice. Alterations in these subsets are evident in older mice following antigenic stimulation.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of these antibodies is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 microL per million cells or 20 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.