PerCP/Cy5.5 anti-mouse CD154
Description
CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
References
1. Lettesjö H, et al. 2000. J. Immunol. 165:4095. (IHC)
2. Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC)
3. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block)
4. Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block)
5. Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366.