PE anti-DYKDDDDK Tag

Antibodies Single
Sony
L5
Flow Cytometry
Rat IgG2a, λ
Mouse
DYKDDDDK-tagged mouse Langerin
3786545
$110.00

Description

The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Park SH, et al. 2008. J Immunol Methods. 331:27.
2. Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
3. Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
4. Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
5. Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
6. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
7. Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed