Brilliant Violet 650™ anti-mouse CD25

Antibodies Single
Sony
PC61
Flow Cytometry
Rat IgG1, λ
Mouse
IL-2-dependent cytolytic mouse T-cell clone B6.1
1110190
$283.00

Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the microg size, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. For immunofluorescent staining using the microL size, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
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11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.