PerCP/Cy5.5 anti-human Ki-67

Antibodies Single
Sony
Ki-67
Flow Cytometry
Mouse IgG1, κ
Human
Nuclei of the Hodgkin lymphoma cell line L428
2352600
$318.00

Description

Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. The antigen Ki-67 interacts with Hklp2, hNIFK, and chromobox protein homolog 1, 3, and 5. Ki-67 is required for cell proliferation and its expression is restricted to the phases G1, S, G2, and M of the cell cycle. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application

References

1. Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
2. Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
3. Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
4. Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
5. Guha P, et al. 2013. PNAS. 110:5052. PubMed