PerCP/Cy5.5 anti-mouse LAP (TGF-β1)

Antibodies Single
Sony
TW7-16B4
Flow Cytometry
Mouse IgG1, κ
Mouse
Mouse <em>Tgfb1</em>-transduced P3U1 cells
1307045
$110.00

Description

Transforming growth factor β (TGF-β) is a cytokine that has critical functions in the immune response by regulating Treg and Th17 cells. TGF-β is first synthesized as pro-TGF-β and then it is cleaved by furin proprotein convertase in the Golgi apparatus to produce the dimeric propeptides called latency-associate peptide (LAP) that non-covalently associates with the dimeric mature TGF-β to prevent its activity. This complex can further associate with latent-TGF-β-binding protein (LTBP) to produce a large latent form for deposition onto the extracellular matrix. The latent-TGF-β can be expressed on the membrane of activated Treg cells, immature dendritic cells, megakaryocytes, and platelets.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

References

1. Oida T, et al. 2010. PLoS One 5:e15523. (FC, IP, WB)
2. Oida T, et al. 2011. PLoS One 6:e18365. (Neut)
3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed