Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 microg per 10⁶ cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)