Alexa Fluor® 488 anti-human CD325 (N-Cadherin)
Description
CD325 (N-cadherin) is a 130 kD, single pass transmembrane protein. Its extracellular region consists of five EC domains and has one cytoplasmic domain. N-cadherin is involved in organogenesis and maintenance of organ architecture by contributing to the sorting of heterogeneous cell types and in the cell adhesion needed to form tissues. N-cadherin is expressed by stem cells, myeloblasts, endothelial cells, and fibroblasts, and also is expressed in neural and muscle tissues and some types of carcinoma cells. CD325 associates with the cytoskeleton trough catenin proteins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
References
1. Navarro P, et al. 1998. J. Cell Biol. 140:1475. (IF)
2. Kim JB. 2000. J. Cell Biol. 151:1193. (Block, WB)
3. Puch S, et al. 2001. J. Cell. Sci. 114:1567. (IF)
4. Wahl JK. 3rd, et al. 2003. J. Biol. Chem. 278:17269. (WB)
5. Wein F, et al. 2010. Stem. Cell Res. 4:129. (FC)
6. Jaggi M, et al. 2002. Cell. Commun. Adhes. 9:103. (IF)