PE anti-mouse PIR-A/B

Antibodies Single
Sony
6C1
Flow Cytometry
Rat IgG1, κ
Mouse
Recombinant extracellular domains 1 and 2 of PIR-A
1320515
$110.00

Description

Paired-immunoglobulin-like receptor (PIR) is a type I transmembrane protein, containing six Ig-like domains. Two isoforms (PIR-A and PIR-B) have been reported for this molecule. PIR-A/B is expressed on B cells, mast cells, macrophages, granulocytes, eosinophils, and dendritic cells. PIR-A requires association with FcRγc for its cell surface expression and to act as an activating receptor. The ligand for PIR-A is MHC class I. PIR-B is an inhibitory receptor with 3 ITIM motifs on its cytoplasmic tail; when phosphorylated, they recruit the tyrosine phosphatases PTPN6/SHP-1 and PTPN11/SHP-2. MHC class I, Nogo, MAG, and OMgp are the ligands for PIR-B.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 microg per million cells in 100 microL volume.  It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Kubagawa H, et al. 1999. J. Exp. Med. 189:309. (WB)
2. Nakamura A, et al. 2004. Nat. Immunol. 5:623. (FC)
3. Masuda K, et al. 2005. EMBO J. 24:4052. (IHC)