Alexa Fluor® 488 anti-human Ki-67
Description
Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. The antigen Ki-67 interacts with Hklp2, hNIFK, and chromobox protein homolog 1, 3, and 5. Ki-67 is required for cell proliferation and its expression is restricted to the phases G1, S, G2, and M of the cell cycle. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies.
Formulation
microg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Recommended Usage
Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining using the microg size, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. For flow cytometric staining using test sizes, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. For immunofluorescence microscopy, a concentration range of 1-5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
References
1. Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
2. Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
3. Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
4. Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
5. Guha P, et al. 2013. PNAS. 110:5052. PubMed