Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the microg size, the suggested use of this reagent is ≤0.125 microg per million cells in 100 microL volume. For flow cytometric staining using the microL size, the suggested use of this reagent is ≤5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
2. Vannucchi AM, et al. 2000. Blood 95:2559.
3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
4. Grisendi S, et al. 2005. Nature 437:147. (FC)
5. Bourdeau A, et al. 2007. Blood 109:4220.
6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
7. Heuser M, et al. 2007. Blood 110:1639. (FC)