APC anti-human HLA-G

Antibodies Single
Sony
87G
Flow Cytometry
Mouse IgG2a, κ
Human
HLA-G transfected cells
2279545
$139.00

Description

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Odum N, et al. 1991. Eur. J. Immunol. 21:2121.
2. Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150.
3. Lila N, et al. 2002. Circulation. 105:1949.
4. Blaschitz A, et al. 2000. Hum. Immunol. 61:1074.
5. Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed