Description

T cell receptor (TCR) is a heterodimer consisting of an α and β chain (TCR α/β) or a γ and δ chain (TCR γ/δ). TCR associates with CD3 to form a CD3/TCR complex.  The CD3/TCR plays a key role in antigen recognition, signal transduction, and T cell activation.  TCR Vγ1.1 (Garman nomenclature) is also called TCR Vγ1 (Tonegawa nomenclature). The Vγ1 gene almost exclusively rearranges to the Jγ4-Cγ4 gene. Vγ1- Jγ4-Cγ4 expressing cells constitute a major population of γ/δ T cells in thymus and peripheral lymphoid organs in adult mice, but they are only composed of a minor population of γ/δ T cells during fetal and early postnatal life. Vγ1 T cell development can happen in thymus-dependent and thymus-independent manners. Further studies have shown that the antibody 2.11 recognized epitote is located in Cr4 domain.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Pereira P, et al. 1995. J. Exp. Med. 182:1921. (IP)
2. Pereira P and Boucontet L. 2004. J. Immunol. 173:3261. (FC)
3. Pereira P, et al. 1996. Intl. Immunol. 8:83. (FC)
4. Dong P, et al. 2014. PLoS Pathog. 9:112964. PubMed