PE anti-mouse CD8b.2

Antibodies Single
Sony
53-5.8
Flow Cytometry
Rat IgG1, κ
Mouse
Mouse thymus or spleen
200 µg
1302040
$214.00

Description

CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
2. Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
3. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
4. Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
5. Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)