Description

The TCR α (α) chain complexes with the TCR β (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 microg per million cells in 100 microL volume or 100 microL of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed