Description

CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. For immunofluorescence microscopy, a concentration range of 5-10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Almagro S, et al. 2010. Mol. Cell Biol. 30:1703. (WB, IF, IP)
2. Zhang F, et al. 2004. J. Biol. Chem. 279:11760. (WB)
3. Iurlaro M, et al. 2004. Am. J. Pathol. 165:181. (IF)
4. Corada M, et al. 2001. Blood 97:1679. (IP, Block)
5. Kooistra M, et al. 2005. FEBS 579:4966. (Block)
6. Corada M, et al. 2001. Blood 97:1679. (Block)
7. Bouillet L, et al. 2013. Laboratory Investigation 93:1194-11202.