Description

Vγ3⁺ T cells are the predominant γδ TCR-bearing cells in early fetal thymus.  The majority of CD3⁺ cells in 14-day fetal thymus express Vγ3.   Vγ3 is also expressed on Thy-1⁺ dendritic epidermal cells (Thy-1⁺ DECs).  Cells that express Vγ3 are not significantly detectable in adult lymphoid organs.  It has been reported that Lck and Fyn, two Src family kinases, are required for the development of Vγ3⁺ T cells.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Havran WL, et al. 1989. P. Natl. Acad. Sci. USA 86:4189. (IP)
2. Havran WL, et al. 1999. J. Immunol. 142:1422. (IF)
3. Uchida Y, et al. 2011. J. Immunol. 186:6945. PubMed
4. Hu S, et al. 2013. J. Immunol. 190:3267. PubMed.