Pacific Blue™ anti-human CD5

Antibodies Single
Sony
UCHT2
Flow Cytometry
Mouse IgG1, κ
Human
2103120
$260.00

Description

CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-Cells), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 microg per 106 cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
2. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC)
3. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437.
4. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed
5. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)