Pacific Blue™ anti-human CD20

Antibodies Single
Sony
2H7
Flow Cytometry
Mouse IgG2b, κ
Human
Human tonsillar B cells
2111640
$278.00

Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Formulation

test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
microg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis.
For test size, the suggested use of this reagent for immunofluorescent staining is 5 microL per 106 cells in 100 microL volume.
For microg sizes, the suggested use of this reagent for immunofluorescent staining is ≤0.5 microg per 106 cells in 100 microL volume.
It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
6. Del Prete GQ, et al. 2014. Antimicrob Agents Chemother. 58:6790. PubMed
7. Li H, et al. 2015. J Infect Dis. 211:1717. PubMed