PE/Cy7 anti-mouse I-A/I-E

Antibodies Single
Sony
M5/114.15.2
Flow Cytometry
Rat IgG2b, κ
Mouse
Activated C57BL/6 mouse spleen cells
1138145
$98.00

Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
2. Viville S, et al. 1993. Cell 72:635. (IHC)
3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
6. Guo M, et al. 1995. Zygote 3:65. (IHC)
7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed