PE anti-mouse TCR Vδ4

Antibodies Single
Sony
GL2
Flow Cytometry
Hamster IgG2, κ
Mouse
G57BL/6 mouse intestinal intra-epithelial lymphocytes
25 µg
1274525
$139.00

Description

Lymphocytes expressing γ/δ antigen receptors constitute a minor population in lymphoid tissue, but a major population within epidermal, gastraintestine, and vaginouterine epithelia. Gamma/Delta T cells are involved in immunological surveillance of epithelia separating the external environment from the internal milieu. The GL2 antibody reacts with Vδ4 T-cell receptor (TCR) bearing T cells, which are the predominant γ/δ T cells in intestinal epithelium (γ/δ IEL). Gamma/delta T cells has been found in mammary glad as well. The frequency of Vδ4 TCR-bearing cells varies among inbred strains of mice.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Lefrancois L, et al. 1990. Cell. 66:33
2. Sperling AI, et al. 1992. J. Immunol. 149(10):3200
3. Pereira P, et al. 1997. Proc. Natl. Acad. Sci. USA. 94:5761