Purified anti-mouse CD3

Antibodies Single
Sony
17A2
Other
Rat IgG2b, κ
Mouse
γδTCR-positive T-T hybridoma D1
1101010
$104.00

Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, CMCD)
2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete)
3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (CMCD)
4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
5. Zan H, et al. 2005. EMBO J. 24:3757.
6. Morgado P, et al. 2011. Infect Immun. 79:4401. PubMed
7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
8. Lei F, et al. 2013. J Vis Exp. 60:3986. PubMed
9. Kopec AK, et al. 2014. Toxicol Sci. PubMed