APC/Cy7 anti-human CD154
Description
CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
References
1. Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF)
2. Rushworth SA, et al. 2002. Transplantation 73:635. (Block)
3. Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF)
4. Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block)
5. Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed
6. Kuchen S, et al. 2007. J. Immunol. 179:5886.
7. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed