Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining . For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 microg per 10⁶ cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.