PerCP/Cy5.5 anti-mouse CD24

Antibodies Single
Sony
M1/69
Flow Cytometry
Rat IgG2b, κ
Mouse
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
1109115
$134.00

Description

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximun emission of 690 nm.

References

1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed